rabbit anti-mouse ccl9 500-p117 Search Results


rabbit anti-mouse ccl9 500-p117  (PeproTech)
90
PeproTech rabbit anti-mouse ccl9 500-p117
Hog barn dust extract increases <t>CCL9</t> expression in RAW264.7. Cells were treated for 1, 3, 6, or 24 h with either media or hog barn dust extract at 1% v/v and measured for CCL9. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. 1 = significant between media treatments, P < 0.05 or lower; 2 = significant between hog barn dust extract treatments, P < 0.05 or lower; 3 = significant between media and hog barn dust extract treatments, P < 0.05 or lower
Rabbit Anti Mouse Ccl9 500 P117, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-mouse ccl9 500-p117/product/PeproTech
Average 90 stars, based on 1 article reviews
rabbit anti-mouse ccl9 500-p117 - by Bioz Stars, 2026-02
90/100 stars
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Hog barn dust extract increases CCL9 expression in RAW264.7. Cells were treated for 1, 3, 6, or 24 h with either media or hog barn dust extract at 1% v/v and measured for CCL9. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. 1 = significant between media treatments, P < 0.05 or lower; 2 = significant between hog barn dust extract treatments, P < 0.05 or lower; 3 = significant between media and hog barn dust extract treatments, P < 0.05 or lower

Journal: Environmental disease

Article Title: Swine barn dust stimulates CCL9 expression in mouse monocytes through PKC-delta activation

doi: 10.4103/ed.ed_16_20

Figure Lengend Snippet: Hog barn dust extract increases CCL9 expression in RAW264.7. Cells were treated for 1, 3, 6, or 24 h with either media or hog barn dust extract at 1% v/v and measured for CCL9. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. 1 = significant between media treatments, P < 0.05 or lower; 2 = significant between hog barn dust extract treatments, P < 0.05 or lower; 3 = significant between media and hog barn dust extract treatments, P < 0.05 or lower

Article Snippet: The membrane was blocked with 5% milk in tris-buffered saline + 0.1% Tween20 (TBST) (0.1% Tween), followed by overnight incubation with 1:1000 dilution of rabbit anti-mouse CCL9 (500-P117, Peprotech, Rocky Hill, NJ) at 4°C.

Techniques: Expressing

CCL9 is produced in response to lipopolysaccharide and peptidoglycan. RAW264.7 cells were treated 6 h with either media, lipopolysaccharide, peptidoglycan, hog barn dust extract (1% v/v), or hog barn dust extract that was boiled for 1 h or scrubbed with polymyxin B. Bars represent CCL9 protein average of 3 replicates performed 3 times. Error bars represent standard error mean. **P < 0.01, ****P < 0.0001

Journal: Environmental disease

Article Title: Swine barn dust stimulates CCL9 expression in mouse monocytes through PKC-delta activation

doi: 10.4103/ed.ed_16_20

Figure Lengend Snippet: CCL9 is produced in response to lipopolysaccharide and peptidoglycan. RAW264.7 cells were treated 6 h with either media, lipopolysaccharide, peptidoglycan, hog barn dust extract (1% v/v), or hog barn dust extract that was boiled for 1 h or scrubbed with polymyxin B. Bars represent CCL9 protein average of 3 replicates performed 3 times. Error bars represent standard error mean. **P < 0.01, ****P < 0.0001

Article Snippet: The membrane was blocked with 5% milk in tris-buffered saline + 0.1% Tween20 (TBST) (0.1% Tween), followed by overnight incubation with 1:1000 dilution of rabbit anti-mouse CCL9 (500-P117, Peprotech, Rocky Hill, NJ) at 4°C.

Techniques: Produced

Protein kinase C-δ inhibitor is involved in production of CCL9. RAW264.7 cells were treated 1 h prior to administration of hog barn dust extract with inhibitors to protein kinase C-α, δ, and ζ. hog barn dust extract was then administered and cells incubated an additional 6 h with inhibitors still present. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. *P < 0.01. NS = No significance

Journal: Environmental disease

Article Title: Swine barn dust stimulates CCL9 expression in mouse monocytes through PKC-delta activation

doi: 10.4103/ed.ed_16_20

Figure Lengend Snippet: Protein kinase C-δ inhibitor is involved in production of CCL9. RAW264.7 cells were treated 1 h prior to administration of hog barn dust extract with inhibitors to protein kinase C-α, δ, and ζ. hog barn dust extract was then administered and cells incubated an additional 6 h with inhibitors still present. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. *P < 0.01. NS = No significance

Article Snippet: The membrane was blocked with 5% milk in tris-buffered saline + 0.1% Tween20 (TBST) (0.1% Tween), followed by overnight incubation with 1:1000 dilution of rabbit anti-mouse CCL9 (500-P117, Peprotech, Rocky Hill, NJ) at 4°C.

Techniques: Incubation

Protein kinase C-δ siRNA blocks CCL9 protein expression. siRNA containing either a nontargeting control (null) or protein kinase C-δ inhibiting sequence (protein kinase C -δ) was transfected into LA4 cells for 24 h prior to treatment of cells with either media or hog barn dust extract for 6 h. Release of CCL9 was measured. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. *P < 0.05, ****P < 0.0001

Journal: Environmental disease

Article Title: Swine barn dust stimulates CCL9 expression in mouse monocytes through PKC-delta activation

doi: 10.4103/ed.ed_16_20

Figure Lengend Snippet: Protein kinase C-δ siRNA blocks CCL9 protein expression. siRNA containing either a nontargeting control (null) or protein kinase C-δ inhibiting sequence (protein kinase C -δ) was transfected into LA4 cells for 24 h prior to treatment of cells with either media or hog barn dust extract for 6 h. Release of CCL9 was measured. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. *P < 0.05, ****P < 0.0001

Article Snippet: The membrane was blocked with 5% milk in tris-buffered saline + 0.1% Tween20 (TBST) (0.1% Tween), followed by overnight incubation with 1:1000 dilution of rabbit anti-mouse CCL9 (500-P117, Peprotech, Rocky Hill, NJ) at 4°C.

Techniques: Expressing, Control, Sequencing, Transfection

CCL9 inhibits stimulated keratinocyte-derived chemokine protein expression. Purified CCL9 was administered (20 ng/mL) to RAW264.7 cells concurrent with lipopolysaccharide, peptidoglycan, or hog barn dust extract treatment and incubated for 6 h. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. *P < 0.05; ****P < 0.0001

Journal: Environmental disease

Article Title: Swine barn dust stimulates CCL9 expression in mouse monocytes through PKC-delta activation

doi: 10.4103/ed.ed_16_20

Figure Lengend Snippet: CCL9 inhibits stimulated keratinocyte-derived chemokine protein expression. Purified CCL9 was administered (20 ng/mL) to RAW264.7 cells concurrent with lipopolysaccharide, peptidoglycan, or hog barn dust extract treatment and incubated for 6 h. Bars represent average of 3 replicates performed 3 times. Error bars represent standard error mean. *P < 0.05; ****P < 0.0001

Article Snippet: The membrane was blocked with 5% milk in tris-buffered saline + 0.1% Tween20 (TBST) (0.1% Tween), followed by overnight incubation with 1:1000 dilution of rabbit anti-mouse CCL9 (500-P117, Peprotech, Rocky Hill, NJ) at 4°C.

Techniques: Derivative Assay, Expressing, Purification, Incubation